{"id":568,"date":"2020-11-16T13:25:50","date_gmt":"2020-11-16T13:25:50","guid":{"rendered":"https:\/\/blogs.ncl.ac.uk\/grahamr\/?p=568"},"modified":"2020-11-18T10:43:36","modified_gmt":"2020-11-18T10:43:36","slug":"new-est-publication-impact-of-redox-conditions-on-antibiotic-resistance-conjugative-gene-transfer-frequency-and-plasmid-fate-in-wastewater-ecosystems","status":"publish","type":"post","link":"https:\/\/blogs.ncl.ac.uk\/grahamr\/2020\/11\/16\/new-est-publication-impact-of-redox-conditions-on-antibiotic-resistance-conjugative-gene-transfer-frequency-and-plasmid-fate-in-wastewater-ecosystems\/","title":{"rendered":"New ES&amp;T Publication: Impact of redox conditions on antibiotic resistance conjugative gene transfer frequency and plasmid fate in wastewater ecosystems"},"content":{"rendered":"\n<p>We are\u00a0happy\u00a0to\u00a0present\u00a0our new publication in\u00a0<em>Environmental Science &amp; Technology<\/em>.\u00a0This <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.est.0c03714\">paper<\/a> is from Mui-Choo (Florence) Jong\u2019s PhD research on antibiotic resistance (AR) gene transfer in low-energy sponge bioreactors. Florence showed sequential redox conditions generally enhanced AR gene removal, but she also observed very different gene transfer frequencies under different conditions. To test this observation, she developed a reporter assay using a green-fluorescent-protein tagged <em>E. coli<\/em> to track conjugative AR plasmid fate; the survival of the <em>E. coli<\/em> host cells; and gene exchange activity in aerobic, anoxic and anaerobic bioreactors, both in biofilms and the liquid phase. Overall, her work, reported in <em>ES&amp;T<\/em>, show that aerobic conditions are better at reducing AR levels and gene transfer in wastewater ecosystems, perhaps due to the reduced host survival and in situ predation. This has major practical implications to wastewater treatment process design.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"594\" height=\"439\" src=\"https:\/\/blogs.ncl.ac.uk\/grahamr\/files\/2020\/11\/microscopy-analysis-1.png\" alt=\"\" class=\"wp-image-571\" srcset=\"https:\/\/blogs.ncl.ac.uk\/grahamr\/files\/2020\/11\/microscopy-analysis-1.png 594w, https:\/\/blogs.ncl.ac.uk\/grahamr\/files\/2020\/11\/microscopy-analysis-1-300x222.png 300w, https:\/\/blogs.ncl.ac.uk\/grahamr\/files\/2020\/11\/microscopy-analysis-1-406x300.png 406w\" sizes=\"auto, (max-width: 594px) 100vw, 594px\" \/><figcaption>Microscopy analysis showing (A &amp; C) Phase contrast, and (B &amp; D) epifluorescence images of food vacuoles expressing Gfp fluorescence suggesting pRP4 host cells potentially engulfed by predatory protozoa. <\/figcaption><\/figure>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>We are\u00a0happy\u00a0to\u00a0present\u00a0our new publication in\u00a0Environmental Science &amp; Technology.\u00a0This paper is from Mui-Choo (Florence) Jong\u2019s PhD research on antibiotic resistance (AR) gene transfer in low-energy sponge bioreactors. Florence showed sequential redox conditions generally enhanced AR gene removal, but she also observed &hellip; <a href=\"https:\/\/blogs.ncl.ac.uk\/grahamr\/2020\/11\/16\/new-est-publication-impact-of-redox-conditions-on-antibiotic-resistance-conjugative-gene-transfer-frequency-and-plasmid-fate-in-wastewater-ecosystems\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":8853,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[34],"tags":[7,54,56,55,19,53],"class_list":["post-568","post","type-post","status-publish","format-standard","hentry","category-publication","tag-antibiotic-resistance","tag-gene-transfer","tag-plasmid-fate","tag-redox-conditions","tag-wastewater","tag-wastewater-treatment-process-design"],"_links":{"self":[{"href":"https:\/\/blogs.ncl.ac.uk\/grahamr\/wp-json\/wp\/v2\/posts\/568","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.ncl.ac.uk\/grahamr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.ncl.ac.uk\/grahamr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/grahamr\/wp-json\/wp\/v2\/users\/8853"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/grahamr\/wp-json\/wp\/v2\/comments?post=568"}],"version-history":[{"count":4,"href":"https:\/\/blogs.ncl.ac.uk\/grahamr\/wp-json\/wp\/v2\/posts\/568\/revisions"}],"predecessor-version":[{"id":577,"href":"https:\/\/blogs.ncl.ac.uk\/grahamr\/wp-json\/wp\/v2\/posts\/568\/revisions\/577"}],"wp:attachment":[{"href":"https:\/\/blogs.ncl.ac.uk\/grahamr\/wp-json\/wp\/v2\/media?parent=568"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/grahamr\/wp-json\/wp\/v2\/categories?post=568"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/grahamr\/wp-json\/wp\/v2\/tags?post=568"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}