{"id":312,"date":"2014-02-24T12:42:59","date_gmt":"2014-02-24T12:42:59","guid":{"rendered":"https:\/\/blogs.ncl.ac.uk\/igmit\/?p=312"},"modified":"2014-02-24T12:42:59","modified_gmt":"2014-02-24T12:42:59","slug":"hpc-lampredi-software-update-fastx-toolkit-0-0-13","status":"publish","type":"post","link":"https:\/\/blogs.ncl.ac.uk\/igmit\/?p=312","title":{"rendered":"HPC &#8211; Lampredi &#8211; software update &#8211; FASTX-Toolkit 0.0.13"},"content":{"rendered":"<p>FASTX-Toolkit 0.0.13 has been added to the cluster (24\/02\/2014)<\/p>\n<p>The FASTX-Toolkit is a collection of command line tools for Short-Reads FASTA\/FASTQ files preprocessing.<br \/>\n<a href=\"http:\/\/hannonlab.cshl.edu\/fastx_toolkit\/\">http:\/\/hannonlab.cshl.edu\/fastx_toolkit\/<\/a><br \/>\n<code><span style=\"font-family: Georgia, 'Times New Roman', 'Bitstream Charter', Times, serif\">-------------------------------------------------------------------<\/span><\/code><\/p>\n<p><code><span style=\"font-family: Georgia, 'Times New Roman', 'Bitstream Charter', Times, serif\">$ module load apps\/fastx<\/span><\/code><\/p>\n<p>apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>| &#8212; libs\/gcc\/system &#8230; SKIPPED (already loaded)<\/p>\n<p>OK<\/p>\n<p>$ ls $FASTXDIR<\/p>\n<p>bin\u00a0 share<\/p>\n<p>ls $FASTXBIN<\/p>\n<p>fasta_clipping_histogram.pl \u00a0 \u00a0 fastx_barcode_splitter.pl<\/p>\n<p>fasta_formatter \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 fastx_clipper<\/p>\n<p>fasta_nucleotide_changer\u00a0 \u00a0 \u00a0 \u00a0 fastx_collapser<\/p>\n<p>fastq_masker\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 fastx_nucleotide_distribution_graph.sh<\/p>\n<p>fastq_quality_boxplot_graph.sh\u00a0 fastx_nucleotide_distribution_line_graph.sh<\/p>\n<p>fastq_quality_converter \u00a0 \u00a0 \u00a0 \u00a0 fastx_quality_stats<\/p>\n<p>fastq_quality_filter\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 fastx_renamer<\/p>\n<p>fastq_quality_trimmer \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 fastx_reverse_complement<\/p>\n<p>fastq_to_fasta\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 fastx_trimmer<\/p>\n<p>fastx_artifacts_filter\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 fastx_uncollapser<\/p>\n<p>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;-<\/p>\n<p>$ module whatis apps\/fastx<\/p>\n<p>apps\/fastx :<br \/>\nTitle: fastx<\/p>\n<p>Summary: A collection of command line tools for Short-Reads FASTA\/FASTQ files preprocessing<\/p>\n<p>License: AGPLv3+<\/p>\n<p>Group: Bioinformatics<\/p>\n<p>URL: http:\/\/hannonlab.cshl.edu\/fastx_toolkit\/<\/p>\n<p>Name: fastx<\/p>\n<p>Version: 0.0.13<\/p>\n<p>Module: apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>Module path: \/opt\/gridware\/etc\/modules\/apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>Package path: \/opt\/gridware\/pkg\/apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>&nbsp;<\/p>\n<p>Repository: git+http:\/\/download.alces-software.com\/git\/packager-base@0d67d87<\/p>\n<p>Package: apps\/fastx\/0.0.13@3f151052<\/p>\n<p>Last update: 2012-09-09<\/p>\n<p>&nbsp;<\/p>\n<p>Builder: root@headnode1.lampredi.cluster.local<\/p>\n<p>Build date: 2014-02-24T10:41:52<\/p>\n<p>Build modules: libs\/gtextutils\/0.6\/gcc-4.4.6<\/p>\n<p>Compiler: compilers\/gcc\/system<\/p>\n<p>System: Linux 2.6.32-279.2.1.el6_lustre.gc46c389.x86_64 x86_64<\/p>\n<p>Arch: Intel(R) Xeon(R) CPU E5620 @ 2.40GHz, 2&#215;4 (b6b89932)<\/p>\n<p>Dependencies: libs\/gcc\/system (using: libs\/gcc\/system)<\/p>\n<p>&nbsp;<\/p>\n<p>For further information, execute:<\/p>\n<p>module help apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;-<\/p>\n<p>$ module help apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>&nbsp;<\/p>\n<p>&#8212;&#8212;&#8212;&#8211; Module Specific Help for &#8216;apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6&#8217; &#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>======== fastx ========<\/p>\n<p>A collection of command line tools for Short-Reads<\/p>\n<p>FASTA\/FASTQ files preprocessing<\/p>\n<p>======================<\/p>\n<p>&nbsp;<\/p>\n<p>This module sets up your environment for the use of the &#8216;fastx&#8217;<\/p>\n<p>application. This module sets up version &#8216;0.0.13&#8217; of the<\/p>\n<p>application.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&gt;&gt; SYNOPSIS &lt;&lt;<\/p>\n<p>&nbsp;<\/p>\n<p>The FASTX-Toolkit is a collection of command line tools for<\/p>\n<p>Short-Reads FASTA\/FASTQ files preprocessing.<\/p>\n<p>&nbsp;<\/p>\n<p>Next-Generation sequencing machines usually produce FASTA or FASTQ<\/p>\n<p>files, containing multiple short-reads sequences (possibly with<\/p>\n<p>quality information).<\/p>\n<p>&nbsp;<\/p>\n<p>The main processing of such FASTA\/FASTQ files is mapping (aka<\/p>\n<p>aligning) the sequences to reference genomes or other databases<\/p>\n<p>using specialized programs. Example of such mapping programs are:<\/p>\n<p>Blat, SHRiMP, LastZ, MAQ and many many others.<\/p>\n<p>&nbsp;<\/p>\n<p>However, it is sometimes more productive to preprocess the<\/p>\n<p>FASTA\/FASTQ files before mapping the sequences to the genome &#8211;<\/p>\n<p>manipulating the sequences to produce better mapping results. The<\/p>\n<p>FASTX-Toolkit tools perform some of these preprocessing tasks.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&gt;&gt; LICENSING &lt;&lt;<\/p>\n<p>&nbsp;<\/p>\n<p>This package is made available subject to the following license(s):<\/p>\n<p>&nbsp;<\/p>\n<p>AGPLv3+<\/p>\n<p>&nbsp;<\/p>\n<p>Please refer to the website for further details regarding licensing.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&gt;&gt; FURTHER INFORMATION &lt;&lt;<\/p>\n<p>&nbsp;<\/p>\n<p>More information about this software can be found at the website:<\/p>\n<p>&nbsp;<\/p>\n<p>http:\/\/hannonlab.cshl.edu\/fastx_toolkit\/<\/p>\n<p>&nbsp;<\/p>\n<p>For further details regarding this module, including the environment<\/p>\n<p>modifications it will apply, execute:<\/p>\n<p>&nbsp;<\/p>\n<p>module show apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&gt;&gt; GET STARTED &lt;&lt;<\/p>\n<p>&nbsp;<\/p>\n<p>Please refer to the website for further details on usage of this<\/p>\n<p>package.<\/p>\n<p>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;-<\/p>\n<p>$ module show apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>\/opt\/gridware\/etc\/modules\/apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6:<\/p>\n<p>module-whatis<\/p>\n<p>&nbsp;<\/p>\n<p>Title: fastx<\/p>\n<p>Summary: A collection of command line tools for Short-Reads FASTA\/FASTQ files preprocessing<\/p>\n<p>License: AGPLv3+<\/p>\n<p>Group: Bioinformatics<\/p>\n<p>URL: http:\/\/hannonlab.cshl.edu\/fastx_toolkit\/<\/p>\n<p>&nbsp;<\/p>\n<p>Name: fastx<\/p>\n<p>Version: 0.0.13<\/p>\n<p>Module: apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>Module path: \/opt\/gridware\/etc\/modules\/apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>Package path: \/opt\/gridware\/pkg\/apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>&nbsp;<\/p>\n<p>Repository: git+http:\/\/download.alces-software.com\/git\/packager-base@0d67d87<\/p>\n<p>Package: apps\/fastx\/0.0.13@3f151052<\/p>\n<p>Last update: 2012-09-09<\/p>\n<p>&nbsp;<\/p>\n<p>Builder: root@headnode1.lampredi.cluster.local<\/p>\n<p>Build date: 2014-02-24T10:41:52<\/p>\n<p>Build modules: libs\/gtextutils\/0.6\/gcc-4.4.6<\/p>\n<p>Compiler: compilers\/gcc\/system<\/p>\n<p>System: Linux 2.6.32-279.2.1.el6_lustre.gc46c389.x86_64 x86_64<\/p>\n<p>Arch: Intel(R) Xeon(R) CPU E5620 @ 2.40GHz, 2&#215;4 (b6b89932)<\/p>\n<p>Dependencies: libs\/gcc\/system (using: libs\/gcc\/system)<\/p>\n<p>&nbsp;<\/p>\n<p>For further information, execute:<\/p>\n<p>module help apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>&nbsp;<\/p>\n<p>module-prereq libs\/gcc\/system<\/p>\n<p>module-conflict apps\/fastx<\/p>\n<p>setenv FASTXDIR \/opt\/gridware\/pkg\/apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6<\/p>\n<p>setenv FASTXBIN \/opt\/gridware\/pkg\/apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6\/bin<\/p>\n<p>setenv FASTXLIB \/opt\/gridware\/pkg\/apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6\/lib<\/p>\n<p>prepend-path GDFONTPATH \/usr\/share\/fonts\/dejavu<\/p>\n<p>setenv GNUPLOT_DEFAULT_GDFONT DejaVuSans<\/p>\n<p>prepend-path PATH \/opt\/gridware\/pkg\/apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6\/bin<\/p>\n<p>prepend-path LD_LIBRARY_PATH \/opt\/gridware\/pkg\/apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6\/lib<\/p>\n<p>\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014\u2014-<\/p>\n<p>Lampredi HPC<br \/>\nigm-lampredi-users@ncl.ac.uk<\/p>\n<p>IGM IT<br \/>\nigmithelp@ncl.ac.uk<br \/>\nx8625<\/p>\n","protected":false},"excerpt":{"rendered":"<p>FASTX-Toolkit 0.0.13 has been added to the cluster (24\/02\/2014) The FASTX-Toolkit is a collection of command line tools for Short-Reads FASTA\/FASTQ files preprocessing. http:\/\/hannonlab.cshl.edu\/fastx_toolkit\/ &#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;- $ module load apps\/fastx apps\/fastx\/0.0.13\/gcc-4.4.6+gtextutils-0.6 | &#8212; libs\/gcc\/system &#8230; SKIPPED (already loaded) OK $ ls $FASTXDIR bin\u00a0 share ls $FASTXBIN fasta_clipping_histogram.pl \u00a0 \u00a0 fastx_barcode_splitter.pl fasta_formatter \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 <a href='https:\/\/blogs.ncl.ac.uk\/igmit\/?p=312' class='excerpt-more'>[&#8230;]<\/a><\/p>\n","protected":false},"author":1149,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[11,10,9,21],"class_list":["post-312","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-cluster","tag-hpc","tag-lampredi","tag-lampredi2","category-1-id","post-seq-1","post-parity-odd","meta-position-corners","fix"],"_links":{"self":[{"href":"https:\/\/blogs.ncl.ac.uk\/igmit\/index.php?rest_route=\/wp\/v2\/posts\/312","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.ncl.ac.uk\/igmit\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.ncl.ac.uk\/igmit\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/igmit\/index.php?rest_route=\/wp\/v2\/users\/1149"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/igmit\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=312"}],"version-history":[{"count":10,"href":"https:\/\/blogs.ncl.ac.uk\/igmit\/index.php?rest_route=\/wp\/v2\/posts\/312\/revisions"}],"predecessor-version":[{"id":333,"href":"https:\/\/blogs.ncl.ac.uk\/igmit\/index.php?rest_route=\/wp\/v2\/posts\/312\/revisions\/333"}],"wp:attachment":[{"href":"https:\/\/blogs.ncl.ac.uk\/igmit\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=312"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/igmit\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=312"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/igmit\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=312"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}