{"id":99,"date":"2015-05-12T18:37:22","date_gmt":"2015-05-12T17:37:22","guid":{"rendered":"https:\/\/blogs.ncl.ac.uk\/katarzynapirog\/?p=99"},"modified":"2016-01-20T18:44:37","modified_gmt":"2016-01-20T18:44:37","slug":"trouble-with-tribbles","status":"publish","type":"post","link":"https:\/\/blogs.ncl.ac.uk\/piroglab\/2015\/05\/12\/trouble-with-tribbles\/","title":{"rendered":"Trouble with Tribbles.."},"content":{"rendered":"<p style=\"text-align: justify\"><span style=\"color: #0000ff\"><strong>TRIB1<\/strong><\/span>\u00a0(Tribbles Pseudokinase 1) is a protein containing a serine\/threonine kinase-like domain which is\u00a0expressed in skeletal muscle, thyroid, pancreas, peripheral blood leukocytes, and bone marrow. Trib1 is critical for the differentiation of tissue-resident macrophages and in musculoskeletal tissues\u00a0it is highly expressed in the bone marrow of\u00a0adult humans. However, its role in skeletal tissues is currently unknown.<\/p>\n<p style=\"text-align: justify\">In collaboration with<span style=\"color: #ff6600\"> <strong><a style=\"color: #ff6600\" href=\"http:\/\/www.ncl.ac.uk\/icm\/people\/profile\/drew.rowan\">Prof Drew Rowan<\/a><\/strong> <\/span>at Newcastle University, we have been analysing a mouse model which overexpresses Trib1 in cartilage (under col2 promoter).<\/p>\n<p><a href=\"https:\/\/blogs.ncl.ac.uk\/katarzynapirog\/files\/2016\/01\/trib1-blog.jpg\" rel=\"attachment wp-att-100\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-100\" src=\"https:\/\/blogs.ncl.ac.uk\/katarzynapirog\/files\/2016\/01\/trib1-blog.jpg\" alt=\"trib1 blog\" width=\"1057\" height=\"404\" srcset=\"https:\/\/blogs.ncl.ac.uk\/piroglab\/files\/2016\/01\/trib1-blog.jpg 1057w, https:\/\/blogs.ncl.ac.uk\/piroglab\/files\/2016\/01\/trib1-blog-300x115.jpg 300w, https:\/\/blogs.ncl.ac.uk\/piroglab\/files\/2016\/01\/trib1-blog-768x294.jpg 768w, https:\/\/blogs.ncl.ac.uk\/piroglab\/files\/2016\/01\/trib1-blog-1024x391.jpg 1024w\" sizes=\"auto, (max-width: 1057px) 100vw, 1057px\" \/><\/a><\/p>\n<p>We have found that <strong><span style=\"color: #0000ff\">mice overexpressing Trib1 in cartilage<\/span><\/strong> (<em>right<\/em>)\u00a0are much smaller than the wild type litter mates (<em>left<\/em>) and present with a pronounced hip dysplasia.\u00a0The bones appear dense, shorter and broader and are\u00a0very reminiscent of <span style=\"color: #0000ff\"><strong>osteopetrosis<\/strong><\/span> (&#8220;the stone bone&#8221; disease).<\/p>\n<p style=\"text-align: justify\"><a href=\"https:\/\/mikebriggs1910.files.wordpress.com\/2015\/05\/trib1-mouse-bones.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-2037\" src=\"https:\/\/mikebriggs1910.files.wordpress.com\/2015\/05\/trib1-mouse-bones.jpg?w=625&amp;h=892\" alt=\"TRIB1 mouse bones\" width=\"625\" height=\"892\" \/><\/a><span style=\"color: #3366ff\"><strong>Jennifer Gerbracht<\/strong><\/span>, an Erasmus student in the lab, generated this bone density image (<em>above<\/em>) using bone mineral content quantification by Faxitron X-ray microradiography. The image was pseudocoloured, showing the densest material in red. The knee joint of a wild type mouse (<em>left<\/em>) is shown compared to a mutant\u00a0 mouse (<em>right<\/em>) with much higher bone density.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>TRIB1\u00a0(Tribbles Pseudokinase 1) is a protein containing a serine\/threonine kinase-like domain which is\u00a0expressed in skeletal muscle, thyroid, pancreas, peripheral blood leukocytes, and bone marrow. Trib1 is critical for the differentiation of tissue-resident macrophages and in musculoskeletal tissues\u00a0it is highly expressed in the bone marrow of\u00a0adult humans. However, its role in skeletal tissues is currently unknown. &hellip; <a href=\"https:\/\/blogs.ncl.ac.uk\/piroglab\/2015\/05\/12\/trouble-with-tribbles\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">Trouble with Tribbles..<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":4483,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-99","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/blogs.ncl.ac.uk\/piroglab\/wp-json\/wp\/v2\/posts\/99","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.ncl.ac.uk\/piroglab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.ncl.ac.uk\/piroglab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/piroglab\/wp-json\/wp\/v2\/users\/4483"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/piroglab\/wp-json\/wp\/v2\/comments?post=99"}],"version-history":[{"count":1,"href":"https:\/\/blogs.ncl.ac.uk\/piroglab\/wp-json\/wp\/v2\/posts\/99\/revisions"}],"predecessor-version":[{"id":101,"href":"https:\/\/blogs.ncl.ac.uk\/piroglab\/wp-json\/wp\/v2\/posts\/99\/revisions\/101"}],"wp:attachment":[{"href":"https:\/\/blogs.ncl.ac.uk\/piroglab\/wp-json\/wp\/v2\/media?parent=99"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/piroglab\/wp-json\/wp\/v2\/categories?post=99"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.ncl.ac.uk\/piroglab\/wp-json\/wp\/v2\/tags?post=99"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}