Cell lines:

• ATDC5
• SW1353
• Tc28a2
• U2OS
• SaOS2
• HT1080

Plasmids:

• pCDNA3.1
• pcDNA3.1 KIF22 ORF-FLAG
• pcDNA3.1 ASPN ORF
• pcDNA-V5His
• pBluescript KS+
• pSF-CMVPuro-NH2-Flag-3C
• pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W
• Lenti-iCas9-neo

Transgenic models:

• Flp
• Col2Cre
• Aspn flp/flp
• Kif22 Pro143Ser

Equipment:

• Faxitron VersaVision Xray machine
• Epredia Shandon Tissue Spin tissue processor
• Epredia Linistat linear stainer
• DunnLabortechnik Flexcell FX-5000 Compression System