Publications (peer-reviewed)

(Please see ResearchGate for open-access articles)

Miotto M, Gouveia RM, Ionescu AM, Figueiredo F, Hamley IW, Connon CJ. 4D Corneal Tissue Engineering: Achieving Time-Dependent Tissue Self-Curvature through Localized Control of Cell Actuators. Advanced Functional Materials 2019, 29(8), 1807334

Ricardo M. Gouveia, Flora Vajda, Jason A. Wibowo, Francisco Figueiredo, Che J. Connon, YAP, ΔNp63, and β-Catenin Signaling Pathways Are Involved in the Modulation of Corneal Epithelial Stem Cell Phenotype Induced by Substrate Stiffness. Cells 2019, 8: 347

Ricardo M. Gouveia, Guillaume Lepert, Suneel Gupta, Rajiv R. Mohan, Carl Paterson & Che J. Connon, Assessment of corneal substrate biomechanics and its effect on epithelial stem cell maintenance and differentiation. Nature Communications 2019,10: 1496 (Nature Communications Editors’ Highlights) (press release)

Che J. Connon and Ricardo M. Gouveia, Autogenous Biofabrication of Nativelike, Scaffold-Free Human Skin Equivalents Using a Smart, Enzyme-Degradable Tissue Templating Coating. ACS Applied Bio Materials 2019, 2: 838–847

Miotto M, Gouveia RM, Abidin FZ, Figueiredo F, Connon CJ, Developing a continuous bioprocessing approach to stromal cell manufacture. ACS Applied Materials & Interfaces 2017, 9: 41131–41142 (press release)

Gouveia, R.M., Koudouna, E., Jester, J., Figueiredo, F., Connon, C.J., Template geometry influences cell alignment to create improved human corneal tissue equivalents. Advanced Biosystems 2017, 1: 1700135 (journal cover image) (radio interview)

Figueiredo, G.S., Bojic, S., Rooney, P., Wilshaw, S-P., Connon C.J., Gouveia, R.M., Paterson, C., Lepert, G., Mudhar, H.S., Figueiredo, F.C., Lako, M., Gamma-irradiated human amniotic membrane decellularised with sodium dodecyl sulfate is a more efficient substrate for the ex vivo expansion of limbal stem cells. Acta Biomaterialia 2017, 61: 124-33

Gouveia, R.M., Gonzalez-Andrades, E., Cardona, J.C., Gonzalez-Gallardo, C., Ionescu, A.M., Garzon, I., Alaminos, M., Gonzalez-Andrades, M., Connon, C.J., Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability. Biomaterials 2017, 121: 209-19 (press release) (BBC News)

Lepert, G., Gouveia, R.M., Connon, C.J., Paterson, C., Assessing corneal biomechanics with Brillouin spectro-microscopy. Faraday Discussions 2016, 187:415-28

Gouveia, R.M. and Connon, C.J., Collagen Scaffolds for Corneal Regeneration. Biomaterials and Regenerative Medicine in Ophthalmology 2nd Edition, T.V. Chirila and D. Harkin (Editors), Woodhead Publishing, Elsevier 2016, pp. 151-78

Kirkham, S., Hamley, I.W., Smith, A.M., Gouveia, R.M., Connon, C.J., Reza, M., Ruokolainen, J., A self-assembling fluorescent dipeptide conjugate for cell labelling. Colloids and Surfaces B: Biointerfaces 2016, 137: 104-8 (journal cover image)

Gouveia, R.M., Hamley, I.W., Connon, C.J., Bio-fabrication and physiological self-release of tissue equivalents using smart peptide amphiphile templates. Journal of Materials Science: Materials in Medicine 2015, 26: 242

Abidin, F.Z., Gouveia, R.M., Connon, C.J., Application of retinoic acid improves form and function of tissue engineered corneal construct. Organogenesis 2015, 11: 122-136

Miotto, M., Gouveia, R.M., Connon, C.J., Peptide Amphiphiles in Corneal Tissue Engineering. Journal of Functional Biomaterials 2015, 6: 687-707

Gouveia, R.M., Castelletto, V., Hamley, I.W., Connon, C.J., New self-assembling multifunctional templates for the biofabrication and controlled self-release of cultured tissue. Tissue Engineering Part A 2015, 21: 1772-1784

Foster, J.W., Gouveia, R.M., Connon, C.J., Low-glucose enhances keratocyte-characteristic phenotype from corneal stromal cells in serum-free conditions. Scientific Reports 2015, 5:10839

Dehsorkhi, A., Gouveia, R.M., Smith, A.M., Hamley, I.W., Castelletto, V., Connon, C.J., Reza, M., Ruokolainen, J., Self-assembly of a dual functional bioactive peptide amphiphile incorporating both matrix metalloprotease substrate and cell adhesion motifs. Soft Matter 2015, 11: 3115-3124

Gouveia, R.M., Jones, R.R., Hamley, I.W., Connon, C.J., The bioactivity of composite Fmoc-RGDS-collagen gels. Biomaterials Science 2014, 2: 1222-29

Foster, J.W., Jones, R.R., Bippes, C.A., Gouveia, R.M., Connon, C.J., Differential nuclear expression of Yap in basal epithelial cells across the cornea and substrates of differing stiffness. Experimental Eye Research 2014, 127: 37-41

Castelletto, V., Gouveia, R., Connon, C.J., Hamley, I.W., Seitsonen, J., Ruokolainen, J., Longo, E., Siligardi, G., Alanine-rich amphiphilic peptide containing the RGD cell adhesion motif: a coating material for human fibroblast attachment and culture. Biomaterials Science 2014, 2: 867-74 

Castelletto, V., Gouveia, R.M., Connon, C.J., Hamley, I.W., Seitsonen, J., Nykanen, A., Ruokolainen, J., Influence of elastase on alanine-rich peptide hydrogels. Biomaterials Science 2014, 2: 867-874

Gouveia R.M. and Connon C.J., The effects of retinoic Acid on human corneal stromal keratocytes cultured in vitro under serum-free conditions. Investigative Ophthalmology & Visual Science 2013, 54: 7483-7491

Gouveia, R.M., Castelletto, V., Alcock, S.G., Hamley, I.W., Connon, C.J., Bioactive films produced from self-assembling peptide amphiphiles as versatile substrates for tuning cell adhesion and tissue architecture in serum-free conditions. Journal of Materials Chemistry B 2013, 1: 6157-6169

Castelletto, V., Gouveia, R., Connon, C.J., Hamley, I.W., New RGD-peptide amphiphile mixtures containing a negatively charged diluent. Faraday Discussions 2013, 166: 381-397

Castelletto, V., Gouveia, R., Connon, C.J., Hamley, I.W., Self-assembly and bioactivity of a polymer/peptide conjugate containing the RGD cell adhesion motif and PEG. European Polymer Journal 2013, 49: 2961-2967

Wang, Y., Loers, G., Pan, H. C., Gouveia, R., Zhao, W. J., Shen, Y. Q., Kleene, R., Costa, J., Schachner, M., Antibody fragments directed against different portions of the human neural cell adhesion molecule L1 act as inhibitors or activators of L1 function. PLoS One 2012, 7 :e52404

Gouveia, R., Schaffer, L., Papp, S., Grammel, N., Kandzia, S., Head, S.R., Kleene, R., Schavhner, M., Conradt, H., Costa, J., Expression of glycogenes in differentiating human NT2N neurons. Downregulation of fucosyltransferase 9 leads to decreased Lewis(x) levels and impaired neurite outgrowth. Biochimica et Biophysica Acta 2012,1820: 2007-2019

Wolterink, S., Moldenhauer, G., Fogel, M., Kiefel, H., Pfeifer, M., Luttgau, S., Gouveia, R., Costa, J., Endell, J., Moebius, U., Altevogt, P., Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer Research 2010, 70: 2504-2515

Gouveia, R., Schaffer, L., Papp, S., Kandzia, S., Head, S.R., Conradt, H., Costa, J., Profiling Human Neuronal Glycosylation: Glyco-Gene Expression and Total N-Glycans from NT2N Neurons. Glycobiology 2010, 20: 1475-1475

Gouveia, R., Kandzia, S., Conradt, H., Costa, J., Production and N-glycosylation of recombinant human cell adhesion molecule L1 from insect cells using the stable expression system. Effect of dimethyl sulfoxide. Journal of Biotechnology 2010, 145: 130-138

Gouveia, R., Kandzia, S., Conradt, H., Costa, J., N-Glycosylation of Human Cell Adhesion Molecule L1 Expressed in Insect Cells using the Stable Expression System Effect of Dimethyl Sulfoxide. Glycobiology 2009, 19: 1364-1364

Gouveia, R.M., Gomes, C.M., Sousa, M., Alves, P.M., Costa, J., Kinetic analysis of L1 homophilic interaction: role of the first four immunoglobulin domains and implications on binding mechanism. Journal of Biological Chemistry 2008, 283: 28038-28047

Gouveia, R.M., Morais, V.A., Peixoto, C., Sousa, M., Regalla, M., Alves, P.M., Costa, J., Production and purification of functional truncated soluble forms of human recombinant L1 cell adhesion glycoprotein from Spodoptera frugiperda Sf9 cells. Protein Expression & Purification 2007, 52: 182-193

Brito, C., Gouveia, R., Costa, J., Stable expression of an active soluble recombinant form of human fucosyltransferase IX in Spodoptera frugiperda Sf9 cells. Biotechnology Letters 2007, 29: 1623-1630

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