Molecular Interplay in Progression of Neuropathic pain and Parkinson’s Disease.

This study aims to identify the differential gene and protein expression associated with pain pathways in iPSCs-derived sensory neurons obtained from Parkinson’s patients or patients with TSC mutations vs iPSCs controls sensory neurons using genetic and proteins analyses. Then determining the functional abnormalities in patients’ iPSC-derived sensory in response to pain stimuli by using functional analyses e.g., calcium imaging, patch-clamp or multi-electrode arrays. By identifying the potentially deregulated proteins in patient models, it will be possible to pinpoint therapeutic targets to reverse the abnormal functional behaviours of patients’ sensory neurons through pharmacological intervention, or by genetic manipulation of the target genes using CRISPR-Cas9 technology.