My Internship at Organon

by Grace Preston, Stage 3 Biomedical Sciences

A Little Context

Hello, I’m Grace, I study Biomedical Science and I had the option to take a placement year away from studying and get into the job role behind my subject. I was one of the lucky few to be offered a year at Organon.

Most of you will not have heard of Organon but once I did my research into the company and its values, I found it to be the perfect option for me.

Organon is a global women’s healthcare company based in 62 countries (to be precise) but lucky for me I was based just around the corner in Cramlington. Different sites vary in testing and research but in Cramlington we focus on testing drugs already on the market (known as stability) and new drugs preparing to be put on the market (known as release).

A Day in the Life of a Lab Intern

After our training we were given the teams we would be based in. I got put into the montelukast team also known as singular (I know, long drug name) which is a drug for asthma specifically for children’s asthma attacks but can be used for lots more. The team were so helpful in getting me up to speed with the number of drugs we receive each week and the typical plan for the week to make sure we release them before their guideline date. I soon learnt each week is different with which type of drug we received and trust me I still can’t name you every single variation of each different drug and how many different tests are needed for them.

My days varied with the different training I received during my year such as UV work, use of DR baths, content uniformity and assays all used to determine how effective the drug is through time points. After a couple months our teams changed and become merged with another drug called Zocor also known as Simvastatin which is for lowering cholesterol.

Me using a high-performance liquid chromatography (HPLC) system.

Is It Challenging?

Of course it is, as is every job. Going from a full-time uni student going out with friends (a little too much I care to admit) and basing your life around lectures and which test or essay is next to a full time 9-5 job is a struggle at first, but you’ll be surprised to know how easy it becomes after a few weeks. The timelines and responsibility were very similar to university, but the stress is so much different at the end of the day you finish work and that’s your day done; not “oh no I need to revise”.

My Advice

If you’re looking for placements look into the companies and job roles so you can understand the expectation and if it suits you. Theres no harm in applying for lots and when you get to the interview stage, practice your questions and even make some of your own for when you get there, and do some research into the company but most importantly just be yourself.

Ps LinkedIn is amazing for connecting with current placement students as well as current placements on offer.

So Should You Do a Placement…?

Simple answer, absolutely! The experience in a scientific working atmosphere is amazing. It helps you gain experience for your future jobs as well as lets you understand your subject so much better and helps you realise what areas you’d like to be based in in later life. I have not only gained so much experience scientifically but also made some incredible friends and gained so much more working life skills that I will 100% be using.

It may be stressful looking at jobs in areas you’ve never even looked at before but trust me just do it, everyone is so welcoming and helpful and you’ll be surprised how much you can learn in just a year.

So, take this as a sign to start placement hunting and get your CVs and cover letters sent in, you won’t regret it!

12th April 202412th April 2024

The wonderful world of Microbiology

Ever wondered what goes on in the Newcastle University Biomedical Sciences labs behind the scenes? On Wednesday 28th February, researchers from the school of Biosciences, Nutritional and Sports Sciences organised a Microbiology research fair to give students a sneak peak into the fascinating world of microbiology research, PhDs, careers, summer placements and final year projects. There were also amazing opportunities to go and see the research laboratories and facilities on the lab tours. So I couldn’t resist tagging along to see what was going on – and let me tell you, it didn’t disappoint! From chatting with researchers deep in ground-breaking projects to getting a first hand look at cutting-edge equipment, there was so much to discover.

Students were talked through different research at each station

There were many areas of research, but one big topic that caught my attention was the gut microbiome. Even though this research fair was mainly aimed at Biosciences students, it was interesting as a student dietitian myself to see the vast amount of research that the University is conducting on this area, as it is a really new and exciting area in the advancing field of nutrition. Some PhD students were researching into how fibre is broken down in the gut by different strains of gut bacteria and how the body uses these chemicals that are produced. One student was focussing particularly on how caramelised sugars are digested by the gut microbiome, and even though excess sugars are seen to have harmful effects on health, some gut bacteria can break these down to useful chemicals!

One of the most interesting stations for me was the station that was showing a machine taking species from the sea to make antibiotics. A remote machine goes down 4000m deep in the South Pacific and takes samples from the bottom of the ocean. It was fascinating how intricate the collection process was, yet how simply the machine could extract the sediment. These samples are then transported back to the labs and the microbes are cultured. The microbes of particular interest to them are Actinomycetes, and these produce 70% of all antibiotics. These actinomycetes have the ability to kill MRSA and other large strains of microbes. These researchers really had a passion for the undiscovered potential of the sea, and it seems like this is a really up and coming area of research.

Here you can see the machine arm working at the bottom of the sea floor to extract the sediment

The most exciting part of the afternoon was the tours of the research labs! From state-of-the-art equipment to cutting-edge facilities, the students got a first hand look at what goes on in the world of research. Our tour leader’s research was focussing on Staphylococcus Aureus. Yes, we are talking about the superbug MRSA – a bacteria that is very complicated as it has the potential to cause lots of different diseases.

Gel electrophoresis equipment in the laboratory

Our fab laboratory tour guide showing us her MRSA superbug cultures

The labs had loads of cool equipment – the second one we were showed around was a biochemistry lab with research focussing on proteins. Their mission is to purify tiny proteins to find the protein of interest, and then grow crystals of it. The coolest machine in the lab by far was the X ray generator, which focuses on the protein crystal. The x ray signal is then amplified and detracts using electron density, which will rotate and shoot as many x rays as possible to collect the data. This helps them to calculate the structure of the molecule with the help of computer software, therefore figuring out what the protein looks like and its function. Ever heard of the lock and key interaction? Well, imagine the protein as the lock and the target molecule as the key. With 3D printing technology, these researchers can create super cool models of the protein structure. And here’s a fun fact to wrap things up – this model below was created of a tyrosine residue. Turns out, it’s not just some fancy scientific term – it’s actually a super important ingredient that can be used in toothpaste to break down plaque in your mouth, showing how important this science research is in everyday life!

The 3D model of tyrosine residues generated by the 3D printer

Biomedical scientists are also actively involved at the Freeman Hospital in researching urinary tract infections (UTIs) through a patient-centred approach, involving collaboration with clinicians ant their patients. This approach emphasizes the importance of understanding the individual needs and experiences of patients in managing and treating UTIs. By working closely with clinicians and patients, these biomedical scientists are contributing to the development of personalized treatment strategies and interventions to improve patients outcomes. They also are conducting laboratory based research to better understand the underlying causes of UTIs, including identification of pathogens, antibiotic resistance patterns and host immune responses to help advance our understanding of them and develop more effective treatment options. This work shows the diverse scope of the Biomedical science profession, which extends beyond traditional lab-based research to the hospitals to use more patient-centred approaches to better improve quality of care.

Judith and Phillip are both active researchers within the Freeman Hospital and with Newcastle University

The research showcased at the fair provided a glimpse into the groundbreaking research happenign at the university in various fields. From the gut microbiome to exploring the potential of the sea for antibiotic discovery, students had so much opportunity to ask questions. The future of science is definitely looking bright!

24th February 202424th February 2024

A year in NHS labs

By Chloe Heppenstall

What goes on in the NHS laboratories?

Rather than a research-based placement, my time in the NHS laboratories involved completing routine assays to test samples and deliver crucial patient results as soon as possible. I was part of the Immunology team within Laboratory Medicine, however there are numerous different departments, such as haematology, virology, microbiology, or chemistry. Having previously worked in the NHS as a domestic, I thought I knew a lot about what happens in the hospitals, but this placement was a huge eye opener as to how much goes on behind the scenes!

Thank you NHS by Karolina Grabowska from Pexels

Why I applied for a placement year.

As I was heading into my second year of University, I was developing a deep interest for Immunology, however I was unsure on what careers would involve this work, and if I would enjoy it. I figured a laboratory-based placement would be the best way for me to understand what work in this sector looks like, and if it was something that I could thrive in. From enjoying my time in the labs at university, I knew a hands on, lab-based placement would be something for me. By taking on this exciting opportunity in the Immunology laboratory in the NHS, I was able to utilise my skill set and knowledge further in an area I was keen on progressing into.

What does a typical day look like?

Each day differed depending on which bench I was allocated to. Within the department, there are 7 different benches, which a Biomedical Scientist would usually spend between 2-6 months on before rotating. These benches involve techniques such as gel electrophoresis, immunofluorescence, immunofixation, immunodisplacement, and automated and manual assays. As part of my placement, I had the opportunity to rotate around all the benches, and each bench was dissimilar to the last. Depending on turnaround times, any backlogs of samples, or any additional work the lab was taking on from other labs, one bench could be a lot busier compared to other benches, and require more staff members to help.

Identifying monoclones via gel electrophoresis]

What did I learn?

In addition to utilising laboratory techniques I acquired during university, I learnt a range of new methods which I will carry as valuable experience for future job prospects. Furthermore, I learnt the importance of turnaround times, and the impact which can occur for the patient if these are not met. My professionalism also grew, as I frequently had to speak to other healthcare professionals, such as nurses in A&E, or midwives. Outside of the lab, the clinical scientists held lectures and journal club sessions, which focussed on the clinical applications and impact of the assays we were completing in the laboratory. This was useful as time in the lab can become very fast paced and repetitive, and it can be easy to forget why you are doing what you are doing. Finally, I made a lot of friends who share similar interests and hobbies to myself, which enabled me to develop a healthy work-life balance.

Me extracting calprotectin samples in a fume cupboard

What did I find challenging?

Although many people warned me, adjusting to the 9-5 working life was extremely difficult. Despite my day finishing at 5 and having my weekends off, I have been just as tired working 9-5 than I was at university with loads of deadlines! On the other hand, there was no stress like there is with university, and if I ever had any problems with work, or I did not understand a concept, the team in Immunology were so welcoming and would give me as much time as I needed to understand and complete something.

Would I recommend a placement to other students?

Absolutely. The skills and experience you acquire whilst on a placement are invaluable, and put you in the best position for applying to jobs after university. If you are seeking hands on experience and a taste of the real world outside of university, do not hesitate to apply to placement opportunities! Aside from the unique skillset I have acquired, I have also been given opportunities to meet new people and discuss their opinions on interesting real-life assay topics, and my potential career in the NHS. I feel confident in a laboratory workplace, which is something I never thought I would be saying!

I now cannot wait to complete my final year at university, obtain my degree, and take my skillset further either in postgraduate studies or in a job.

13th February 202413th February 2024

Would your blood glow?

By Sarah Holder

The Beginning

All the way back at the start of my second year at Newcastle University, the thought of a placement year was a nice one, but I never thought I’d be one of the lucky few to be offered one! When I came across the advert for the job at Labcorp Huntingdon, I knew it would be the only intern position I wanted to apply for. The role sounded perfect and it was located only half an hour from my home village. I sent off my initial application and then received an invite for an interview! And then another! And another! I couldn’t believe it when I received a phone call in January to say I had been offered the intern position for Immunology and Immunotoxicology 2022-2023. Brilliant!

Background to my Project

Human blood is made up of several major components including plasma, platelets, red blood cells and white blood cells. White blood cells are involved in the body’s response to infection and need to be tested separately to the rest of the blood components to see the best results.

Infections can come in the form of viruses or bacteria. Viruses present markers called epitopes on their surface which the white blood cells use to identify what they are. Bacteria don’t directly present these epitopes but via white blood cells they infect which present their epitopes for them. When these epitopes are come across in the bloodstream, specific white blood cells release chemicals called cytokines to activate the immune response and fight the infection. We were adding epitopes artificially in the form of our stimulants to elicit a response from six different human donors and judge the stimulant that gives the clearest response across the six blood samples.

My Project

My project during my time on placement at Labcorp had two parts. Improving the white blood cell isolation method and investigating white blood cell responses to positive controls we added to them and attaching fluorescent green and red markers to the different cytokines produced. We had longer to plan my project than expected so it didn’t get into the lab until May, creating more pressure for everything to go smoothly once it was underway. If anything had gone drastically wrong, we wouldn’t have had enough time to order the reagents required for another attempt. The images taken at the end were full of fluorescent spots so the project worked!

My project has provided a future method for FluoroSpot assays to be carried out at Labcorp on our Huntingdon site which will hopefully be able to bring more revenue into I&I. Future research into the method we developed for my project could be done to optimise it further and make it a more streamlined process.

Life on Placement

I was lucky enough to not only secure a placement but secure a placement I could commute to from my home. Thankfully, my parents were happy to have me back for another year! While the 6:45am wakeup call wasn’t my favourite, I could spend my evenings visiting my friends from home, which was lovely. I worked a mixture of 8am-4pm and 8:30am-4:30pm throughout the week and it was nice actually being able to catch a glimpse of daylight in the winter when leaving at 4pm instead of both arriving and leaving in what feels like the dead of night every day.

Every month a department social was held by the I&I Social Committee. My favourites were the Halloween bar crawl, Christmas party and lab getaway weekend to a lakeside cabin with the team. These really helped me to feel part of the team during my time working at Labcorp.

Summary

I would really recommend a placement year to anyone considering one, the lab experience gained is a perfect CV addition for life after university and the additional skills of working in a team and independently while in an industry setting are invaluable. I was very fortunate and had the best line manager in terms of supporting my transition into a working environment, this was aided massively by the chocolate that magically appeared on my desk every Friday. I’m not sure what my future after university is going to be yet, but thanks to my placement year I know I can join a workplace and adapt to fit in very quickly, and training can be provided if I feel I have any gaps in my knowledge for my future role. No question is a stupid question!

1st February 20245th February 2024

A Placement Year at GSK – Sequencing Genomes

By: Natalie Taylor – BSc Biomedical Sciences

Edited by: Laila Hussain

Second year of uni rolled around, and I realised I hardly had any lab experience (thanks COVID). This was a dilemma I attempted to remedy by applying for a placement year. I never considered I’d be accepted by such a prestigious company as GSK. Even less did I consider I would be working in a field as interesting as genetics. The application stress paid off! I was placed in the Discovery Genomics department working on single cell RNA sequencing. This basically means reading genes that are currently ‘switched on’ (expressed). Knowing this information is crucial for understanding disease, as we can quantify which genes were switched on/off in disease states compared to healthy states. With this information we can then get an idea of the genes function and thus, what therapeutic action to take.

My Application Journey – It’s ok not to know everything!

When I say I was almost 100% sure I failed my interview I was not joking. GSK’s interview stage was split into two sections. One half was situational questions such as “tell us a time you faced a challenge” etc. The other was technical questions. This was where I flopped. I didn’t know the answer to any of the questions! However, I discovered the point of these questions was not only to test your scientific knowledge, this can be taught, but to also see how you manage uncertainty and to test your resilience. It was a daunting experience, but thanks to the kind interviewers and some persistence I managed to give all the answers a go. This was what the interviewers were looking for!

Two days later I received a call from one of the interviewers offering me the placement!

My-day-to-day

As I mentioned above, I’m working on sequencing (or reading) the genes of individual cells. This means I’m in the lab a lot! I get to work on many different diseases and cell types, which all involve working with very small volumes. Attention to detail is paramount – something I’ve had to learn the hard way!

**A visual example of the types of tubes and volumes we typically work with in RNA sequencing- sometimes even smaller!** *Image from: unsplash*

I also get to use lots of very cool – and very expensive – machines. The machine pictured below on the left is what sequences RNA and the one to the right is a robot that can do a big portion of the lab work involved with preparing the RNA for sequencing for you. It has some limitations though, so I mostly performed my experiments manually by hand.

**Illumina Sequencer (Left) & Chromium Connect (Right):** *Photos from 10x Genomics and Illumina*

After about three months of training, I started planning and carrying out my own experiments independently. I got the chance to sequence many different immune cells, including T cells and macrophages as part of active experiments in the search for potential cancer therapeutics. Apart from single cell RNA sequencing, I’ve also been fortunate to perform a CRISPR knock out experiment on B cells whilst using cutting-edge automation platforms such as the Dragonfly and Bravo.

On a typical day, my experiments take around 6 hours to reach a safe stopping point. Luckily, I’m able to get a few breaks in when my samples are incubating. During these breaks, I take full advantage of all of the green space at GSK Stevenage. It’s quite normal for employees to go on regular walks around the site – breaks are very encouraged which is nice!

**GSK Campus and Labs in Stevenage:** *Photos from GSK’s Website*

Outside of lab work, I’ve also gotten involved with the wider community at GSK by being a part of a committee for students called IP Unite and an employee resource group called Women’s Leadership Initiative (WLI). My role in these groups varies, typically I will plan and host events aimed to foster new relationships and grow networks. One of the scariest events I held was a PhD information event with IP Unite that involved me speaking in front of 100 people in a lecture theatre – luckily I was only introducing speakers and closing the event – still, it was very outside of my comfort zone! In general, I find that the placement pushes me outside of my comfort zone, but that’s what yields the most growth so I know it’s super beneficial.

Overall, the placement has been the best experience, better than I expected! I have grown so much as a scientist and a person. I feel much more prepared for life after uni. For now though, I’m just ready to tackle final year!

8th March 202221st March 2022

The Legacy of Henrietta Lacks

By Evangelia Rakou Stage 2 Biomedical Sciences Student

Who is Henrietta Lacks?

It might come as a surprise to you that one of the people who changed the course of medical research was not even a scientist herself. Henrietta Lacks was an African American woman and mother of five who died from cervical cancer in 1951.

After complaining of vaginal bleeding, she was diagnosed with the disease and sadly passed away several months after her diagnosis. However, her cells continue to impact the world and revolutionise modern medicine years after her death.

8th March 202221st March 2022

Dr Marie Maynard Daly: The Mother of Genetics and Heart Diseases

By Luisa Roa Gil 3rd year Physiological Sciences student

Illustration of Dr Marie Maynard Daly by Matteo Farinella on Tumblr

You might expect to instantly recognise the name of someone that contributed to the discovery of DNA structure, revealed the cause of high blood pressure, and became the first African-American woman to obtain a chemistry PhD, right?

However, you may be shocked by how many do not know the story of Dr Marie Maynard Daly – a woman who made multiple advances in science and opened doors for young scientists.

20th April 202121st March 2022

International Women’s Day Blog Competition

We recently asked our students to submit entries detailing the work of inspirational female scientists as part of a blog competition for International Women’s Day. We are delighted to share all of the entries below – choosing the top two was not an easy decision, so congratulations to all writers!

Ada Lovelace – by Olivia Rowe, 3rd year MSci Biochemistry (1st prize)

What does it mean to be a woman?

For centuries, women have been objectified and designated ‘The Second Sex’. Lord Byron’s 19^th century poem ‘She Walks In Beauty’ is a prime example, where he describes his female subject to be as provocative as ‘starry skies’ on a clear night.

22nd January 202121st March 2022

Mary Seacole: A Scientist by Nature

In October, as part of Black History Month in the UK, we ran a competition asking students to submit blog posts showcasing the contribution of scientists of African and Caribbean descent to the scientific world. In the run-up to February’s Black History Month in North America, we are delighted to share the winning blog by Cerys Francis-Garside, Stage 1 Master of Dietetics student.

Mary Seacole: A Scientist by Nature

Perhaps on first hearing her name, you would not choose to label her a scientist. Perhaps you might think “Oh I’ve heard of her… who is she again?”. To me, the story of Mary Seacole is one of the most important in science as it is one we can continue to learn from again and again.

8th March 201921st March 2022

Celebrating International Women’s Day – Women in Science Blog Competition

Women make, and have made, vital contributions to science. This is a statement that should not need to be said, but too often women have not received the credit they deserve.

This year to highlight the achievements of Women in Science we ran a blog competition in the School of Biomedical Sciences. The challenge was to write a blog to highlight the contribution women have made to science.

The 2019 winner was Lilla Marshall (2nd year pharmacology), receiving £50. The close runner up was Caitlin Cosimini (Stage 3 Biomedical Sciences), congratulation to both, here is Lillia’s blog.

Lilla Marshall, winner of the ‘Women in Science’ blog competition 2019

Three Interesting Tales of Women in Science

By Lilla Marshall

Historically, science has been dominated by men. Since the year 2000, only 12.7% of Nobel Prizes for Physiology and Medicine have been awarded to women.

I wasn’t the best in my stats module last semester, but even I can see the problem there. Even in popular culture, if you asked the general public to name as many female scientists as they could – the majority would say Marie Curie and “that lady who had her work on DNA stolen” (meaning Rosalind Franklin).

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